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KMID : 0903520010440030143
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2001 Volume.44 No. 3 p.143 ~ p.147
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Rapid Determination of Degree of Hydrolysis for Hemoglobin by Osmometry
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김동호/Kim, Dong Ho
강인규/채희정/인만진/오남순/Kang, In Kyu/Chae, Hee Jeong/In, Man Jin/Oh, Nam Soon
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Abstract
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An osmometrical method for determination of the degree of hydrolysis (DH) of hemoglobin was comparatively examined through TNBS (trinitrobenzene sulfonic acid) method using two experimental variables (α and β), which were chosen based on correlation curves between hydrolysis equivalents (h) and leucine-NH₂ equivalents. DH values measured through osmometry and TNBS method highly correlated with R² values of 0.974∼0.991, irrespective of the reaction pHs and types of enzyme used. DH_(osm)/DH_(TNBS) was 1.438∼1.656 depending on the hydrolysis pH (7.5∼10.0). Correlation equations were well fit for measuring DH of Hb hydrolysate at different pH conditions. DH_(osm)/DH_(TNBS) for co-treatment system using Esperase and Flavourzyme was 1.658, in good agreement with that of 1.656 for the single enzyme (Esperase) system. Thus, the osmometrical method was suggested to be a convenient, reliable, and rapid method for the determination of DH of hemoglobin hydrolysates.
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